Why is Sanger confirmation needed?

To reduce NGS error risk before clinical reporting.

Is Sanger used for whole-genome analysis?

No, it is intended for limited regions.

Genetic Tests

Sanger sequencing remains the gold standard for NGS variant confirmation and focused single-gene testing.

Dideoxy chain-termination sequencing suited to short DNA fragments.

Confirmation of NGS-identified variants, small gene analysis and clone sequencing.

Extracted DNA, plasmid or PCR product.

Targeted regions defined by specific primers.

Accurate base-by-base sequence of the target region.

Throughput limited to a few hundred bases per reaction.

To Be Added

To Be Added

Confirmation of variants linked to Mendelian disorders.

Contact Gene Negar Ayandegan for sample submission, laboratory collaboration, or more information.